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Association between CPS1 and the TGF-β signaling pathway in CRC (A) Volcano plot based on DESeq2 differential expression analysis (CPS1 high vs. CPS1 low; CPS1 low, n = 238; CPS1 high, n = 237). (B and C) Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. BP, biological process; CC, cellular component; MF, molecular function. (D–F) Correlations between CPS1 and TGFB1, SMAD2, and <t>SMAD3</t> expression ( n = 367). (G and H) Western blotting analysis of TGFB1, SMAD2, p -SMAD2, SMAD3, and p -SMAD3 in LoVo and SW480 cells. Spearman correlation analysis was used in (D)–(F).
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Association between CPS1 and the TGF-β signaling pathway in CRC (A) Volcano plot based on DESeq2 differential expression analysis (CPS1 high vs. CPS1 low; CPS1 low, n = 238; CPS1 high, n = 237). (B and C) Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. BP, biological process; CC, cellular component; MF, molecular function. (D–F) Correlations between CPS1 and TGFB1, SMAD2, and SMAD3 expression ( n = 367). (G and H) Western blotting analysis of TGFB1, SMAD2, p -SMAD2, SMAD3, and p -SMAD3 in LoVo and SW480 cells. Spearman correlation analysis was used in (D)–(F).

Journal: iScience

Article Title: CPS1 promotes colorectal cancer progression by inducing EMT and activating TGF-β/Smad signaling pathway

doi: 10.1016/j.isci.2026.117129

Figure Lengend Snippet: Association between CPS1 and the TGF-β signaling pathway in CRC (A) Volcano plot based on DESeq2 differential expression analysis (CPS1 high vs. CPS1 low; CPS1 low, n = 238; CPS1 high, n = 237). (B and C) Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. BP, biological process; CC, cellular component; MF, molecular function. (D–F) Correlations between CPS1 and TGFB1, SMAD2, and SMAD3 expression ( n = 367). (G and H) Western blotting analysis of TGFB1, SMAD2, p -SMAD2, SMAD3, and p -SMAD3 in LoVo and SW480 cells. Spearman correlation analysis was used in (D)–(F).

Article Snippet: The antibodies included: CPS1 (1:200; A24810 , ABclonal), ZEB1 (1:1000; A5600, ABclonal), Slug (1:1000; A13352, ABclonal), E-cadherin (1:1000; A20798, ABclonal), N-cadherin (1:1000; A3045, ABclonal), Vimentin (1:1000; A19607, ABclonal), MMP2 (1:1000; A19080, ABclonal), TGFB1 (1:1000; A25313, ABclonal), SMAD2 (1:1000; A19114, ABclonal), p -SMAD2 (1:1000; AP1342, ABclonal), SMAD3 (1:1000; A16913, ABclonal), p -SMAD3 (1:1000; AP0727, ABclonal), and GAPDH (1:10000; A19056, ABclonal).

Techniques: Quantitative Proteomics, Functional Assay, Expressing, Western Blot

Journal: iScience

Article Title: CPS1 promotes colorectal cancer progression by inducing EMT and activating TGF-β/Smad signaling pathway

doi: 10.1016/j.isci.2026.117129

Figure Lengend Snippet:

Article Snippet: The antibodies included: CPS1 (1:200; A24810 , ABclonal), ZEB1 (1:1000; A5600, ABclonal), Slug (1:1000; A13352, ABclonal), E-cadherin (1:1000; A20798, ABclonal), N-cadherin (1:1000; A3045, ABclonal), Vimentin (1:1000; A19607, ABclonal), MMP2 (1:1000; A19080, ABclonal), TGFB1 (1:1000; A25313, ABclonal), SMAD2 (1:1000; A19114, ABclonal), p -SMAD2 (1:1000; AP1342, ABclonal), SMAD3 (1:1000; A16913, ABclonal), p -SMAD3 (1:1000; AP0727, ABclonal), and GAPDH (1:10000; A19056, ABclonal).

Techniques: Recombinant, SYBR Green Assay, Lysis, Modification, CCK-8 Assay, Immunohistochemistry, Gene Expression, Plasmid Preparation, Software